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Biotechnology and Applied Biochemistry (2005) 41, (85–88) (Printed in Great Britain)

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Development of an individual-specific autoantibody (ISA) protein microarray

Stewart J. Lebrun¹, Edgar Suquilanda and Wasinee N. Petchpud

Miragene Inc. (formally Miragen), 5475 E. La Palma Ave, Anaheim, CA 92807, U.S.A.

Key words: autoantibody, identification method, individual-specific autoantibody (ISA), meat tracking, protein array, self-antigen.

Abbreviation used: ISA, individual-specific autoantibody.

¹To whom correspondence should be addressed (email lebrun@miragene.com).

We have developed an ISA (individual-specific autoantibody) identification method to identify biological samples based on an individual's unique class of autoantibodies. This method involved the presentation of human proteins derived from crude lysates after SDS/PAGE separation and transferance to a solid support. In the present study, ISA strips are produced and developed on a new protein microarray. In making the ISA strips, it was found that variation in protein migration during electrophoresis and strip-manufacturing quality control limit the reliability of the assay. Therefore it was decided to semi-purify and separate proteins by column chromatography in large batches and to develop an ISA-specific protein microarray. It was found that this ISA protein microarray approximates a similar serum titre as the ISA strip and is predicted to circumvent the batch-to-batch production issues related to SDS/PAGE.

Received 17 February 2004/11 May 2004; accepted 20 May 2004

Published as Immediate Publication 20 May 2004, DOI 10.1042/BA20040026

© 2005 Portland Press Ltd