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Biotechnology and Applied Biochemistry (2008) 50, (1–9) (Printed in Great Britain)
Preparation and in vitro analysis of microencapsulated live Lactobacillus fermentum 11976 for augmentation of feruloyl esterase in the gastrointestinal tract
Jasmine Bhathena, Arun Kulamarva, Christopher Martoni, Aleksandra Malgorzata Urbanska and Satya Prakash1
Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering and Artificial Cells and Organs Research Centre, Faculty of Medicine, McGill University, 3775 University Street, Montreal, QC, Canada H3A 2B4
Key words: ferulic acid (FA), feruloyl esterase (FAE), gastrointestinal (GI) tract, Lactobacillus fermentum, microencapsulated bacteria, oral delivery. Abbreviations used: APA, alginate–PLL–alginate; cfu, colony-forming units; CWW, cell wet weight; DMF, dimethylformamide; EFA, ethyl ferulate; GI, gastrointestinal; FA, ferulic acid; FAE, feruloyl esterase; MRS, Man–Rogosa–Sharpe; PLL, poly(L-lysine); PS, physiological saline; SIF, simulated intestinal fluid. 1To whom correspondence should be addressed (email satya.prakash@mcgill.ca). FA (ferulic acid) is a well-known phenolic phytochemical present in plant cell walls. Various studies have indicated that FA has many physiological functions in the prevention of chronic disease. It has been shown to play an important chemoprotective role in degenerative diseases. FA also shows strong antioxidant and nitrite-scavenging potential and anticarcinogenic and antiinflammatory properties. The in vivo physiological importance of FA depends on its availability for absorption. Dietary fibre-bound FA is partially released by gut micro-organisms; however, the concentration of the released FA is too low to act as a chemopreventive agent. Therefore it is important to augment the bioavailability of FA to appreciate more fully its real physiological effect. This paper evaluates the suitability of the alginate–poly(L-lysine)–alginate microcapsules for oral delivery of live feruloyl esterase-producing Lactobacillus fermentum 11976 cells, in vitro, by using a dynamic simulated human GI (gastrointestinal) model. The present study shows that microencapsulated L. fermentum 11976 cells can efficiently break down a FA-containing substrate, and establishes the biotechnological basis for their use in supplementing the bioavailability of dietary FA in the intestine. Received 5 January 2007/8 June 2007; accepted 20 July 2007 Published as Immediate Publication 20 July 2007, doi:10.1042/BA20070007 © 2008 Portland Press Ltd |
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