| About the journal | | | Subscriptions | | | Authors | | | Users | | | Librarians | | | Readers | | | FAQs |
 |
Biotechnology and Applied Biochemistry (2010) 55, (191–197) (Printed in Great Britain)
Cyclic mechanical strain promotes transforming-growth-factor-β1-mediated cardiomyogenic marker expression in bone-marrow-derived mesenchymal stem cells in vitro
Suk Ho Bhang*1, So-Jung Gwak†1, Tae-Jin Lee†, Sang-Soo Kim*, Ho Hyun Park‡, Moon Hyang Park‡, Dae-Hee Lee§, Soo-Hong Lee§ and Byung-Soo Kim*2
*School of Chemical and Biological Engineering, Seoul National University, San 56-1, Sillim-dong, Gwanak-gu, Seoul 151-744, Republic of Korea, †Department of Bioengineering, Hanyang University, Haengdang-dong, Seongdong-gu, Seoul 133-791, Republic of Korea, ‡Department of Pathology, College of Medicine, Hanyang University, Haengdang-dong, Seongdong-gu, Seoul 133-791, Republic of Korea, and §CHA Stem Cell Institute & CHA Biotech, Pochon CHA University, 605 Yeoksam, Gangnam, Seoul 135-081, Republic of Korea
Key words: bone-marrow-derived mesenchymal stem cell (BMMSC), cardiomyogenic marker expression, cyclic mechanical strain, transforming growth factor-β1 (TGF-β1). Abbreviations used: BMMSC, bone-marrow-derived mesenchymal stem cell, MHC, myosin heavy chain, FBS, fetal bovine serum; MEF, myocyte enhancer factor, MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide; RT–PCR, reverse transcription–PCR, TGF-β1, transforming growth factor-β1. 1These authors contributed equally to this work. 2To whom correspondence should be addressed (email byungskim@snu.ac.kr). Cardiomyocytes in the heart reside in mechanically dynamic environments, such as those subject to cyclic mechanical strain. TGF-β1 (transforming growth factor-β1) stimulates cardiomyogenic marker expression of BMMSCs (bone-marrow-derived mesenchymal stem cells). In the present study, we tested the hypothesis that cyclic mechanical strain promotes TGF-β1-mediated cardiomyogenic marker expression in BMMSCs in vitro. The mRNA expression of cardiac-specific genes was more up-regulated in BMMSCs cultured with a TGF-β1 supplement and subjected to cyclic strain for 1 week than in BMMSCs cultured statically with a TGF-β1 supplement. Immunocytochemical analyses and flow cytometric analysis showed that the proportions of cardiac troponin-I-positive cells and cardiac MHC (myosin heavy chain)-positive cells and the proportions of cells expressing tropomyosin respectively were increased to a greater extent by TGF-β1with cyclic strain than by TGF-β1 alone. These results showed that cyclic strain promotes TGF-β1mediated cardiomyogenic marker expression in BMMSCs in vitro. Received 2 November 2009/22 February 2010; accepted 5 March 2010 Published as Immediate Publication 5 March 2010, doi:10.1042/BA20090307 © 2010 Portland Press Limited |
Bookmark with:
 
|
PDF
My BAB toolbox
