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Most downloaded papers

The papers listed below are the most frequently downloaded papers for the 12 months to the end of April 2009

Downloads Title / Authors
825 Maize (Zea mays) genetic transformation by co-cultivating germinating seeds with Agrobacterium tumefaciens
Jingxue Wang, Yi Sun and Yi Li
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772 Review
Oligosaccharides, neoglycoproteins and humanized plastics: their biocatalytic synthesis and possible medical applications
Jozef Nahálka, Jun Shao and Peter Gemeiner
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672 Erythropoietic and anti-obesity effects of Garcinia cambogia (bitter kola) in Wistar rats
Kayode Alaba Oluyemi, Idowu O. Omotuyi, Olusegun R. Jimoh, Olamide A. Adesanya, Chia L. Saalu and Sunday J. Josiah
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388 Selection and characterization of an internalizing epidermal-growth-factor-receptor antibody
Xiaorong Zhao, Wentao Dai, Limin Cao, Huifen Zhu, Yihan Yu, Qing Ye, Min Wang, Wei Dai, Ping Lei and Guanxin Shen
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387 Studies of thermostability in Camelus bactrianus (Bactrian camel) single-domain antibody specific for the mutant epidermal-growth-factor receptor expressed by Pichia
Kobra Omidfar, Mohhamad Javad Rasaee, Soheila Kashanian, Malieheh Paknejad and Zahra Bathaie
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371 Growth and production of cholesterol oxidase by alginate-immobilized cells of Rhodococcus equi No. 23
Yu-Chih Chang and Cheng-Chun Chou
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365 A new scaleable freeze–thaw technology for bulk protein solutions
Parviz A. Shamlou, Lawrence H. Breen, Willis V. Bell, Mark Pollo and Beth Ann Thomas
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354 Cashew-tree (Anacardium occidentale L.) exudate gum: a novel bioligand tool
Raquel da Silveira Nogueira Lima, Jacira Rabelo Lima, Celio Ribeiro de Salis and Renato de Azevedo Moreira
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339 Ginkgo biloba leaf extract (EGb 761) diminishes adriamycin-induced hyperlipidaemic nephrotoxicity in rats: association with nitric oxide production
Mohamed F. Abd-Ellah and Amr D. Mariee
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299 Small-scale immunopurification of cytochrome c oxidase for a high-throughput multiplexing analysis of enzyme activity and amount
James Murray, Birgit Schilling, Richard H. Row, Chris B. Yoo, Bradford W. Gibson, Michael F. Marusich and Roderick A. Capaldi
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293 Review
Bioreactor systems for the production of biopharmaceuticals from animal cells
James N. Warnock and Mohamed Al-Rubeai
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267 Minireview
Growth of hairy-root cultures in various bioreactors for the production of secondary metabolites
Bhartendu Nath Mishra and Ritu Ranjan
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266 Review
Colorimetric protein assay techniques
Christine V. Sapan, Roger L. Lundblad and Nicholas C. Price
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250 High-level expression of human thyroid-stimulating hormone in Chinese hamster ovary cells by co-transfection of dicistronic expression vectors followed by a dual-marker amplification strategy
Cibele N. Peroni, Carlos R. J. Soares, Elizabeth Gimbo, Ligia Morganti, Maria Teresa C. P. Ribela and Paolo Bartolini
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242 Production of plasmid DNA encoding human hepatocyte growth factor for gene therapy
Qinglin Zhang, Jianjing Bi, Fengjun Xiao, Haifeng Duan, Bing Wu and Zuze Wu
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231 A peroxidase isoenzyme secreted by turnip (Brassica napus) hairy-root cultures: inactivation by hydrogen peroxide and application in diagnostic kits
Elizabeth Agostini, Josefa Hernández-Ruiz, Marino B. Arnao, Silvia R. Milrad, Horacio A. Tigier and Manuel Acosta
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207 Identification of an oxidative refolding intermediate of recombinant consensus interferon from inclusion bodies and design of a two-stage strategy to promote correct disulfide-bond formation
Yong-Dong Liu, Gui-Feng Zhang, Jing-Jing Li, Jing Chen, Yin-Jue Wang, Hong Ding and Zhi-Guo Su
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205 Review
Biomaterials and scaffold design: key to tissue-engineering cartilage
Joanne Raghunath, John Rollo, Kevin M. Sales, Peter E. Butler and Alexander M. Seifalian
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198 Transcriptome and proteome analysis of antibody-producing mouse myeloma NS0 cells cultivated at different cell densities in perfusion culture
Britta Krampe, Halina Swiderek and Mohamed Al-Rubeai
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177 Oligo-1,6-glucosidase from a thermophile, Bacillus thermoglucosidasius KP1006, was efficiently produced by combinatorial expression of GroEL in Escherichia coli
Kunihiko Watanabe1, Hisashi Fujiwara, Kazuyo Inui and Yuzuru Suzuki
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175 Review
Freeze-drying of proteins: some emerging concerns
Ipsita Roy and Munishwar Nath Gupta
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170 Catalytic activities of intracellular dimeric neopullulanase on cyclodextrin, acarbose and maltose
Kyung-A Cheong, Tae-Jip Kim, Jong-Won Yoon, Cheon-Seok Park, Tae-Soo Lee, Young-Bae Kim, Kwan-Hwa Park and Jung-Wan Kim
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169 Review
Recombinant production of antimicrobial peptides in heterologous microbial systems
Aaron B. Ingham and Robert J. Moore
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168 Escherichia coli minimum genome factory
Hiroshi Mizoguchi, Hideo Mori and Tatsuro Fujio
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167 Detection and quantification of affinity ligand leaching and specific antibody fragment concentration within chromatographic fractions using surface plasmon resonance
Pranavan Thillaivinayagalingam, Anthony R. Newcombe, Kieran O'Donovan, Richard Francis and Eli Keshavarz-Moore
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150 Modelling and advanced understanding of unsteady-state gas transfer in shaking bioreactors
Ghassem Amoabediny and Jochen Büchs
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Vol.53 (4): Aug 09
Vol.53 (3): Jul 09
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