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Most downloaded papers

The papers listed below are the most frequently downloaded papers for the 12 months to the end of June 2008

Downloads Title / Authors
433 Review
Metabolomics as a complementary tool in cell culture
Soo Hean Gary Khoo and Mohamed Al-Rubeai
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411 Review
Colorimetric protein assay techniques
Christine V. Sapan, Roger L. Lundblad and Nicholas C. Price
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373 Small-scale immunopurification of cytochrome c oxidase for a high-throughput multiplexing analysis of enzyme activity and amount
James Murray, Birgit Schilling, Richard H. Row, Chris B. Yoo, Bradford W. Gibson, Michael F. Marusich and Roderick A. Capaldi
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327 Review
Oligosaccharides, neoglycoproteins and humanized plastics: their biocatalytic synthesis and possible medical applications
Jozef Nahálka, Jun Shao and Peter Gemeiner
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280 Review
Bioreactor systems for the production of biopharmaceuticals from animal cells
James N. Warnock and Mohamed Al-Rubeai
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269 Review
Recombinant production of antimicrobial peptides in heterologous microbial systems
Aaron B. Ingham and Robert J. Moore
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258 Identification of an oxidative refolding intermediate of recombinant consensus interferon from inclusion bodies and design of a two-stage strategy to promote correct disulfide-bond formation
Yong-Dong Liu, Gui-Feng Zhang, Jing-Jing Li, Jing Chen, Yin-Jue Wang, Hong Ding and Zhi-Guo Su
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249 Review
Biomaterials and scaffold design: key to tissue-engineering cartilage
Joanne Raghunath, John Rollo, Kevin M. Sales, Peter E. Butler and Alexander M. Seifalian
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242 Detection and quantification of affinity ligand leaching and specific antibody fragment concentration within chromatographic fractions using surface plasmon resonance
Pranavan Thillaivinayagalingam, Anthony R. Newcombe, Kieran O'Donovan, Richard Francis and Eli Keshavarz-Moore
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238 Selection and characterization of an internalizing epidermal-growth-factor-receptor antibody
Xiaorong Zhao, Wentao Dai, Limin Cao, Huifen Zhu, Yihan Yu, Qing Ye, Min Wang, Wei Dai, Ping Lei and Guanxin Shen
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215 Comparative expression of different antibody formats in mammalian cells and Pichia pastoris
Ingke Braren, Kerstin Greunke, Oliver Umland, Susanne Deckers, Reinhard Bredehorst and Edzard Spillner
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212 A new scaleable freeze–thaw technology for bulk protein solutions
Parviz A. Shamlou, Lawrence H. Breen, Willis V. Bell, Mark Pollo and Beth Ann Thomas
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196 Maize (Zea mays) genetic transformation by co-cultivating germinating seeds with Agrobacterium tumefaciens
Jingxue Wang, Yi Sun and Yi Li
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180 Studies of thermostability in Camelus bactrianus (Bactrian camel) single-domain antibody specific for the mutant epidermal-growth-factor receptor expressed by Pichia
Kobra Omidfar, Mohhamad Javad Rasaee, Soheila Kashanian, Malieheh Paknejad and Zahra Bathaie
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178 Escherichia coli minimum genome factory
Hiroshi Mizoguchi, Hideo Mori and Tatsuro Fujio
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178 Erythropoietic and anti-obesity effects of Garcinia cambogia (bitter kola) in Wistar rats
Kayode Alaba Oluyemi, Idowu O. Omotuyi, Olusegun R. Jimoh, Olamide A. Adesanya, Chia L. Saalu and Sunday J. Josiah
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174 Review
Nutrigenomics and nutrigenetics: the 'omics' revolution in nutritional science
Edwin C. M. Mariman
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138 Review
Computer-aided tissue engineering: overview, scope and challenges
Wei Sun, Andrew Darling, Binil Starly and Jae Nam
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137 Mini Review
Bacillus minimum genome factory: effective utilization of microbial genome information
Katsutoshi Ara, Katsuya Ozaki, Kouji Nakamura, Kunio Yamane, Junichi Sekiguchi and Naotake Ogasawara
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135 Differences in the glycosylation profile of a monoclonal antibody produced by hybridomas cultured in serum-supplemented, serum-free or chemically defined media
J. Antonio Serrato, Vanessa Hernández, Sandino Estrada-Mondaca, Laura A. Palomares and Octavio T. Ramírez
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132 Expression and characterization of human growth hormone–Fc fusion proteins for transcytosis induction
Chang Hoon Lee, Jung Hee Woo, Kwang Keun Cho, Seung Ha Kang, Sang Kee Kang and Yun Jaie Choi
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122 Minireview
Growth of hairy-root cultures in various bioreactors for the production of secondary metabolites
Bhartendu Nath Mishra and Ritu Ranjan
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122 Review
Genetic design for facilitated production and recovery of recombinant proteins in Escherichia coli
Per Jonasson, Sissela Liljeqvist, Per-åke Nygren and Stefan Ståhl
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120 Review
Freeze-drying of proteins: some emerging concerns
Ipsita Roy and Munishwar Nath Gupta
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118 Review
Dendrimers and their applications in immunoassays and clinical diagnostics
Pratap Singh
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116 A genome-wide transcriptional analysis of producer and non-producer NS0 myeloma cell lines
Soo Hean Gary Khoo, Francesco Falciani and Mohamed Al-Rubeai
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115 An automated microscale chromatographic purification of virus-like particles as a strategy for process development
Marc D. Wenger, Peter Dephillips, Colleen E. Price and Daniel G. Bracewell
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112 Review
Tissue-engineered skin substitutes: from in vitro constructs to in vivo applications
François A. Auger, François Berthod, Véronique Moulin, Roxane Pouliot and Lucie Germain
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110 Running-buffer composition influences DNA-protein and protein-protein complexes detected by electrophoretic mobility-shift assay (EMSA)
Karim Roder and Michael Schweizer
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109 Review
Biotechnological applications for surface-engineered bacteria
Henrik Wernérus and Stefan Ståhl
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109 Cashew-tree (Anacardium occidentale L.) exudate gum: a novel bioligand tool
Raquel da Silveira Nogueira Lima, Jacira Rabelo Lima, Celio Ribeiro de Salis and Renato de Azevedo Moreira
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109 Minireview article
Schizosaccharomyces pombe minimum genome factory
Yuko Giga-Hama, Hideki Tohda, Kaoru Takegawa and Hiromichi Kumagai
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106 A semi-empirical mathematical model useful for describing the relationship between carbon dioxide, pH, lactate and base in a bicarbonate-buffered cell-culture process
Michael J. Gramer and Tad Ogorzalek
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106 Violacein: properties and biological activities
Nelson Durán, Giselle Z. Justo, Carmen V. Ferreira, Patrícia S. Melo, Livia Cordi and Dorival Martins
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106 Review
Lipase assays for conventional and molecular screening: an overview
Rani Gupta, Pooja Rathi, Namita Gupta and Sapna Bradoo
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104 Review
Advances in microbial amylases
Ashok Pandey, Poonam Nigam, Carlos R. Soccol, Vanete T. Soccol, Dalel Singh and Radjiskumar Mohan
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100 Expression of human coagulation Factor IX in transgenic tomato (Lycopersicon esculentum)
Hui Zhang, Lingxia Zhao, Yuhui Chen, Lijie Cui, Weiwei Ren and Kexuan Tang
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99 Inducible Escherichia coli fermentation for increased plasmid DNA production
Aaron E. Carnes, Clague P. Hodgson and James A. Williams
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99 Review
Towards molecular farming in the future: using plant-cell-suspension cultures as bioreactors
Rainer Fischer, Neil Emans, Flora Schuster, Stephan Hellwig and Jürgen Drossard
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96 Comparative studies on the thermostability of five strains of transmissible-spongiform-encephalopathy agent
Karen Fernie, Philip J. Steele, David M. Taylor and Robert A. Somerville
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96 Minireview
The cryopreservation of human embryonic stem cells
Boon Chin Heng, Lilia L. Kuleshova, Soren Müller Bested, Hua Liu and Tong Cao
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94 Ginkgo biloba leaf extract (EGb 761) diminishes adriamycin-induced hyperlipidaemic nephrotoxicity in rats: association with nitric oxide production
Mohamed F. Abd-Ellah and Amr D. Mariee
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91 Modelling and advanced understanding of unsteady-state gas transfer in shaking bioreactors
Ghassem Amoabediny and Jochen Büchs
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91 Review article
The biology and engineering of stem-cell control
Analeah O'Neill and David V. Schaffer
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90 Review
Glycosylation of Pichia pastoris-derived proteins
Roger K. Bretthauer and Francis J. Castellino
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89 A novel PEGylation of chitosan nanoparticles for gene delivery
Yanqiong Zhang, Jiji Chen, Yangde Zhang, Yifeng Pan, Jingfeng Zhao, Lifeng Ren, Mingmei Liao, Zhiyuan Hu, Lu Kong and Jiwei Wang
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88 Human adult craniofacial muscle-derived cells: neural-cell adhesion-molecule (NCAM; CD56)-expressing cells appear to contain multipotential stem cells
Andrea C. M. Sinanan, Nigel P. Hunt and Mark P. Lewis
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87 Interactive effects of growth factors and three-dimensional scaffolds on multipotent mesenchymal stromal cells
Leslie Heckmann, Jörg Fiedler, Thomas Mattes, Martin Dauner and Rolf E. Brenner
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86 Secretory expression and characterization of insulin in Pichiapastoris
Thomas Kjeldsen, Annette FrostPettersson and Morten Hach
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86 Phage passage after extended processing in small-virus-retentive filters
Scott Lute, Mark Bailey, Jessica Combs, Muppalla Sukumar and Kurt Brorson
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